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Plasma aliquots will be thawed on ice and mixed with ethanol/BHT and vortexed.
The serum aliquots will be placed into supplied microtubes containing an anti-protease.
Then, three 10 ml aliquots will be coded and frozen at -20°C for 8-oxodG analysis.
Another up to 100 ml aliquots will be coded and frozen at -20°C for organic extraction and genotoxicity tests.
Aliquots of different types of specimens will be colour coded and the aliquots will be stored in the -80°C freezers within 3 hours of collection.
The aliquot barcodes and biobank database will ensure that the storage position and usage of all aliquots will be able to be tracked.
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A fresh aliquot will be used each time AOM is needed to avoid repeated freeze-thaw cycles.
One aliquot will be spread over a glass slide, to be fixed and stained with Diff Quick (Sigma-Aldrich, São Paulo, Brazil).
The second aliquot will be treated with 0.1% of dithiothreitol (Sigma-Aldrich, USA) and stirred using a vortex mixer for total and differential cell counts.
A 10 ml aliquot will be used to determine renal clearance of cefazolin in the two-hour time interval with the balance of urine used to calculate 2-hour urinary creatinine clearance.
Aliquots of plasma will be separated, labeled and stored at −80 degrees Celsius.
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