Sentence examples for aliquots in a from inspiring English sources

The absorbance of the eluted stain was measured in 200 μL aliquots in a microplate reader at A595 nm and related with an Alizarin Red S standard curve of 0-10 mM sub-dilutions in 10% CPC.

An overnight culture of strain MR-1 in Luria Broth (LB) diluted to an approximate optical density (Optical Density- OD600) of 0.02 was used as a starting culture and distributed into 200 ml aliquots in a 96-well plate.

GFP reporter assays were performed by diluting twice sub-cultured bacteria (OD595 ∼0.3) to OD595 ∼ 0.1 in DTA medium and dispensing 200 µl culture aliquots in a 96-well microplate.

Free and membrane-bound ligand were separated by rapid filtration of 500 µl aliquots in a cell harvester (Brandel, Gaithersburg, MD, USA) through Whatman GF/C filters embedded in 0.3% polyethylenimine that were subsequently washed for 5 s with 5 ml of ice-cold 50 mM Tris-HCl buffer.

The tubes' contents were dispensed as 200 µl aliquots in a 96-well microplate, incubated upto 5 days and the contents plated on Middlebrook 7H11 containing 10% ADC (0.5 g/l BSA, 2g/l Dextrose and 0.085 g/l NaCl) (MB) agar for CFU analysis.

The PRP was stored in 500 μl aliquots in a −70 °C freezer.

Biomass concentrations in cell suspensions were determined as milligram dry weight (mgdw) by drying the aliquot in a pre-weighed aluminium foil container to constant weight at 85 °C overnight.

For amplification, a cDNA aliquot in a volume of 12.5 μl containing 20 mM Tris/HCl (pH 8.4), 50 mM KCl, 1.6 mM MgCl2, 0.4 mM dNTPs, 0.04 unit of Taq DNA polymerase (Gibco-BRL, Carlsbad, CA, U.S.A).

Dissolve 125.1 mg of solid N-ethylmaleimide in 1 ml of 100% ACN, dispense into 4-µl aliquots, store in a desiccator at −20 °C, and use within two months.

A 14-μg protein aliquot in buffer A was supplemented with 100 ng of elastase or trypsin respectively, using stock solutions of 0.1 mg/ml protease in 20 mM Tris/HCl, pH 7.8.

The suspension was distributed in 500ul aliquots in 1.5 ml tubes using a 23 gauge needle and incubated on ice for 5 minutes.