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The ejaculates (n = 10) were divided into two aliquots: first aliquot was treated with CLC (3 mg/120 × 106 sperm), whereas, second aliquot remained untreated and served as control.
The centrifugal transfer (1300 g for 1 min) from the donor to the acceptor MMV was reversed just after the first transfer, resulting in the generation of an MMV in which a very small aliquot remained.
Ten millilitres of the sample was divided into two aliquots of 5 ml each; one aliquot was incubated at 50°C in a water bath for 30 minutes (treated sample) and the second aliquot remained at room temperature (non-treated sample).
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It indicated that antioxidant activity of aliquots of TA remained stable, so transgenic hairy root lines P3, T12 and PT18 showed higher total antioxidant activity of TA due to higher total TA content they produced.
An aliquot was removed as control and the remaining aliquot was treated with hydrogen peroxide to a final concentration of 20 mM.
One aliquot was used for biochemical analysis, and remaining aliquots were used for LC-MS analysis of lipids.
For the remaining aliquot, after the salinity increase the pH was lowered to 5.0 ± 0.1 using 20 μL aliquots of 0.1 M HNO3 ("pH dropped" samples) and the suspension agitated for 24 hours.
To assess whether selection for somatic alleles also occurs in vitro, a remaining aliquot of the earliest cryopreserved immortalized cells was placed in culture.
The remaining aliquot (30 μL) was mixed with 120 μL of sterile PBS.
The remaining aliquot was frozen at -20°C for a measurement of the total protein concentration (BCA; Pierce, Rockford, IL).
An aliquot of the remaining non-sorted emulsion was also plated and used as reference in all subsequent measurements.
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