Figure 2 TEM images of reaction aliquots: (a) sample A at stage 1; (b) sample A at stage 2; (c) sample A at stage 3; (d) sample B at stage 1; (e) sample B at stage 2; (f) sample B at stage 3; (g) sample C at stage 2; (h) sample C at stage 3; (i) sample D at stage 3. Sample B is prepared by reduction at low temperature to prevent continuous seed formation.
The exact hot:cold 18F-7B 7A 18F-7B 7Aas measuration each experiment by counting an aliquot of a sample (2 μL) of the radioligand preparation in a scintillation counter.
A 10-μl aliquot of a sample was collected and mixed with 100 μl of the H2O MeCN HCOOH (50:50:0.1) mixture and ESI-MS measurements were performed immediately.
The semi-automated hydrodynamic sample injection is accomplished via a specially designed PMMA interface that is able to repeatedly inject sample aliquots from a sample volume as low as 10 μL, with repeatability of peak areas below 5%.
Several 200-nL aliquots of a sample were produced and spiked with internal synthetic standard, the physiologically active peptide cerebrin, 20) at the appropriate concentration.
A sample aliquot was removed, APC added (2.0 nM), aliquots taken at selected time points and quenched into denaturing sample preparation buffer, and subsequently analyzed by Western blotting.
A sample aliquot was removed, APC added (2.0 or 20.0 nM), aliquots taken at selected time points and quenched into denaturing sample preparation buffer, and subsequently analyzed by Western blotting.
Afterward, a sample aliquot was cooled on crushed ice for 15 min.
Each FA in a sample aliquot was identified by comparing its retention time against the FAME STDs mixture.
Cross-sections of the samples were made by mounting a sample aliquot in polyester resin "Clear Casting AM" (orthophthalic, acrylic modified Clear Casting Resin, UK).
Input DNA was purified from a sample aliquot equal to 10% of the total cells used for each ChIP.
