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Amino acid sequence alignment of DHQases were performed with MUSCLE (Edgar 2004), and the graphic display of alignments were made by ESPript3.0 (Gouet et al. 1999).
Multiple alignments were made in ClustallW2.
Pairwise alignments were made by using the sequences obtained from forward and reverse primers.
Manual adjustments of the alignments were made when necessary using the BioEdit program [22].
Multiple sequence alignments were made using ClustalW [134], and phylogenetic reconstructions were made using SplitsTree [135].
Multiple sequence alignments were made with ClustalW within the MEGA4 software.
All alignments were made using MEGA 4. Phylogenetic trees were constructed using MrBayes software version 3.0b4 [41].
AVID alignments were made
Two big alignments were made.
Minor adjustments to the alignments were made manually.
Gene alignments were made using ClustalW within MEGA5 [ 46].
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