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Amino acid sequences were aligned using MUSCLE [ 96] and nucleotide alignments were created by forcing the DNA sequence onto the protein alignment using CLUSTALW [ 97].
Various MWCNT alignments were created by changing operating conditions.
The sequences were edited using Bioedit, and multiple sequence alignments were created by using 16S rRNA sequences belonging to our strains and different bacterial species from the NCBI GenBank database for the purpose of developing a phylogenetic tree.
Multiple alignments were created by using ClustalX (2.0.10) (14 ).
DNA sequence alignments were created by using DNASIS Pro Hitachi Solutionss, Tokyo, Japan).
Alignments were created by the implemented ClustalW algorithm, using the substitution BLOSUM weight matrix and the following parameters.
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The human-mouse alignment was created by aligning both genomes using blastz [ 104].
Multiple alignment was created by aligning all sequences against Hidden Markov Model GTP_EFTU from Pfam [ 105] using program HMMALIGN [ 18].
For each DNA sequence, a multiple alignment was created by Clustal W Thompsonn et al. 1994).
Additionally, a combined alignment was created by concatenating our amino acid and nucleotide datasets into a single matrix.
A second sequence alignment was created by adding 12 sequences from GenBank with known dates of collection to the original Nigerian sequences.
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