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In an initial step, the alignments were assessed with GBLOCKS [ 103] and T-coffee.
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Sequence alignments and percentage of amino acid conservation were assessed with the Clustal-W multiple alignment algorithm.
Bootstrap values were assessed with 1000 replicates.
Each individual alignment was assessed for congruence with respect to a combined matrix including unambiguously aligned regions of every gene.
The sagittal alignment and tibial slope are assessed with a lateral image and compared to pre-operative imaging.
Once alignment was assessed and necessary balancing with trial implants was in place, the navigation arrays were removed.
The quality of the alignment was assessed by blending or flickering between the adjacent images.
After applying the ring, external fixator alignment was assessed and corrected.
After applying the ring fixator alignment was assessed and corrected if needed.
The quality of the structural alignment is assessed by the value of DaliLite Z-score, which is a structural similarity score normalized with respect to protein domain size.
The preoperative knee alignment was assessed by the HKA angle.
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