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The accuracy of automatic alignments was confirmed by visual inspection.
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Sequences were aligned within Sequencher 4.8 and alignments were confirmed within MEGA 5.2 [ 47] using ClustalW with default settings.
atpsβ alignments were confirmed by eye and trimmed to 252 bp.
The alignments were confirmed by applying PCA.
We removed alignments with frame shifts and the remaining alignments were confirmed visually.
The alignments were confirmed by visual inspection and potential ORFs were identified using ORFpredictor[ 86] and ORFfinder [ 89].
Opossum CES2.2 and CES2.3 genomic alignments were confirmed using Ensembl (Ensembl release 45, MonDom5 assembly, June 2007) [ 57, 58].
The core sequences of FGFs from different organisms were aligned using the Clustal W program, and the alignment was confirmed manually.
DNA sequences were aligned by ClustalW (Thompson et al. 1994), and alignment was confirmed by MUSCLE (Edgar 2004) and manual inspection.
Another positive phase alignment was confirmed from 140° to 220° at 1.5 2.0 s.
The first sequence from the alignment was confirmed by three sequencing results.
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