Next, is a description of the extensive data collection effort that utilized differential cinematic GPS surveys to define the horizontal alignment variables, and road safety inspections (RSIs) to quantify the other road characteristics related to safety.
Differential densities of alignments suggest variable levels of agricultural intensity.
Alignments of variable positions are provided in supporting information figures (Figure S1, S2, S3, S4).
Like Bockhorst et al. have shown for the DBL2X domain [24], we have developed an expanded alignment to study local alignments of variable blocks from DBL6ε domains.
Alignments containing variable sequence lengths or having low alignment scores were manually evaluated and edited, using BioEdit software [ 31], as previously described [ 18].
As measuring the effect of some of these factors is a complicated issue and beyond the scope of this work, alignments of variable read and reference lengths were used in comparing reads misalignments of Eland, RMAP and Slider.
While comparative studies of protein evolution focus mostly on conserved sequence blocks in multiple sequence alignments (MSAs), variable length regions and the insertion/deletions (indels) they harbor have provided unique insight into how proteins function [ 1- 5] and evolve [ 6- 8].
Although the characteristic TIRs and TSDs of the five β-amylase elements are easy to align, the regions between the TIRs (excluded from Fig. 1 alignment) are variable in both length and sequence (Fig. 2).
All three programs gave a similar overall alignment between human and chimpanzee 10,000 bp sequences, however, it was not possible to obtain a consistent alignment within Variable Regions with the exception of some small segments.
The primers were designed, by multiple sequence alignment of variable regions, specific for cold-adapted donor virus HTCA-A101, as compared to other influenza A viruses.
