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Long-read aligners such as BWA-SW (Li and Durbin, 2010) and AGILE (Misra et al., 2011) severely restrict the number of alignments under consideration early in query processing.
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By default, during the pairwise alignment phase, fragments under consideration are extended until their average substitution score is at least 4 for amino acids (note that our BLOSUM62 matrix has 0 for the lowest score possible) and 0.25 for nucleotides, respectively.
RSS is measured by U-values, which indicate the likelihood that the mutation rate changes for each alignment position between the subfamilies under consideration.
If a reporter has alignments in the reverse direction, but also has at least one high-quality forward alignments to a RefSeq RNA whose status is more definite than "model", then the reverse alignments are ignored and the reporter remains under consideration for being fully valid or RefSeq RNA valid.
However, for the 3-user scenario under consideration, closed-form and computationally efficient solutions do exist for spatial interference alignment in the frequency domain but not in the time domain [1].
We propose a methodology for the pairwise comparison and alignment of metabolic pathways that aims at providing the largest conserved substructure of the pathways under consideration.
However, alignment errors and template selection are still the main bottleneck for current template-base modeling methods, especially when proteins under consideration are distantly related.
> -wrap-foot> We repeated the same tests for every pair of species under consideration such that members in the pair belong to different domains giving rise to four pairwise alignment instances per metabolism.
What's under consideration?
More rules are under consideration.
Mensah is another under consideration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com