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The percent sequence identity with the C. crescentus XylD is presented for the four XylD homologs used and was obtained by aligning the protein sequences using the multiple sequence alignments tool Clustal Omega developed at the European Bioinformatics Institute (EMBL-EBI) (McWilliam et al. 2013).
Annotated potato loci (PGSC0003DMG40*******) corresponding to the genes were obtained using the BLAST sequence alignments tool in (http://potato.plantbiology.msu.edu/integrated_searches.shtml).shtml
Sites diverging between panTro3 and hg19 were obtained using the regional variation/fetch substitutions from the pairwise alignments tool.
A mathematical measure of entropy was determined for each amino acid position using the Shannon Heterogeneity In Alignments Tool V.1.0 (http://evolve.zoo.ox.ac.uk/software).ac.uk/software
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Each 36 bp read was first shortened to 32 bp (a requirement for the alignment tool) and aligned to the grape reference genome [24] using Illumina's ELAND alignment tool.
The Bowtie2 (a.k.a. Bowtie version 2) alignment tool can align reads of very different length.
Consensus sequences were then aligned using MUSCLE alignment tool with default parameters in GENEIOUS PRO.
The trimmed sequences from each barcode bin were aligned using the NAST alignment tool (http://greengenes.lbl.gov) [32].
Finally, we performed a comparison between MP-Align and the SubMAP alignment tool [ 18].
Sequences were aligned using the multiple sequence alignment tool ClustalW [ 38].
Motif-based alignment tool consists of two main parts to align multiple sequences with different affinity scores of STAT TFBSs.
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