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By performing three independent alignments using the staggered genomic indices and then superimposing the resulting alignments to show all aligning sequences, the enhanced-BLAT (eBLAT) detects as much as 75% more conserved sequences when evolutionary distant sequences are aligned [22].

By performing three independent alignments using the staggered indices with the optimized alignment parameters and then superimposing the resulting alignments to show all aligning sequences, the overall detection of conserved sequences has been improved by as much as 75% when evolutionary distant orthologous sequences are aligned.

The parameters for BlastN program were as follows: Expected (E) threshold = 10, comparison matrix = Blosum62, alignments to show = 100, allow gaps = yes, filter = yes.

A random sequence of equal length and composition was included in all alignments to show pairwise percent identities that are not significantly different from random identity.

The default statistical parameters used in the BLASTP analysis were as follows: BLASTP-protein query of the protein database; expected threshold (E): −1, comparison matrix: BLOSUM62; no. of alignments to show: 100.

Further settings were chosen as follows: cost to open a gap = 25, cost to extend a gap = 10, reward for a nucleotide match = 2, penalty for a nucleotide mismatch = -3, word size = 56, megablast search = T, number of alignments to show = 1.

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Sequence alignment to show conserved K2 in human γ-crystallin variants.

A sequence logo was generated at the bottom of the alignment to show the frequency of certain amino acid substitution at a position, indicated by the height of the letter [ 66 ].

Probes with transcript- or gene-specific alignments were expected to show higher signal intensities in an experiment due to more complete template hybridization compared to non-perfect match, no-match, or negative control probes.

Core genome gene alignments were used to show that Azobacter vinelandii clusters closely with other Pseudomonas species, suggesting it may belong to the Pseudomonas genus [ 89].

Gene-EST alignments were required to show at least a 90% identity across 100 bp to be recorded as a match and the number of BLASTN hits were used as a proxy for expression levels.

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