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Trimmed unmapped reads are aligned and used in conjunction with previous alignments to identify chimeric events by examining exon junctions from the annotation file.
We performed a new round of alignments to identify the coding region (CDS) of each mapped transcript.
We manually inspected multiple sequence alignments to identify common sites of the 18S rDNA : large insertions occurring in some sequences were excluded from the alignment to get consistent divergence estimate across pairwise comparions.
bThe software uses alignments to identify the longest fragment, which it deems as full-length.
Methods such as RECON [ 17] utilize whole-genome alignments to identify repetitive elements de novo.
MuTect (version 1.1.4) was applied on the matched tumor-control alignments to identify somatically acquired point mutations.
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Next, the reads at each CAL are further aligned using gapped local alignment to identify the best match.
These sequences were aligned using Clustal Multiple Alignment to identify conserved and variable regions suitable for primers and probes that could differentiate the species.
We then used MuMMer 2.3[ 31]to align the contig sets for each strain, using an all-against-all alignment to identify contigs that were similarly constructed between the assemblers.
As an example, we have analyzed the tetratricopeptide repeat (TPR) motif, using multiple sequence alignment to identify the significance of each position in the TPR.
Here we develop a new WD40 Repeat Recognition method (WDRR), which uses predicted secondary structure information to generate candidate repeat segments, and further employs a profile profile alignment to identify the correct WD40 repeats from candidate segments.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com