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We used local alignments to determine placement of the TSS in all paralogs.
Additionally, we applied the Shimodaira-Hasegawa toseveralexampleexalignmentsnmento to determine whether or not the improvement in goodness-of-fit was influenced by sampling variability.
We have also applied the CUSP algorithm [13] on each of the 353 domain superfamily alignments to determine the locations, typical lengths and preferred structural types of length insertions amongst related domain superfamily members.
However, unlike previous studies that relied on amino acid alignments to determine cleavage sites [33], we obtained additional evidence that these sites were recognised by 2Apro and 3Cpro by using a neural network protease cleavage-site prediction tool.
In this way, we identified the PUF motifs in the PUM1 and PUM2 IPed transcripts (targets) and in the expressed transcripts that were not IPed (non-targets), and we used transcript-to-genome alignments to determine the genomic coordinates of the PUF motifs.
We checked manually the alignments to determine if these copies were true paralogs or could represent artifacts of EST assembly.
Similar(46)
The single-scale cylinder pairs are horizontally orientated and investigated in both horizontal and vertical alignment to determine if optimal configurations exist which maximize heat dissipation at the local cylinder and global array levels.
Subsequently, we performed multiple sequence alignment to determine the corresponding residues for the other 10 COX, and calculated 5 Z-scales for these residues with the function AADescs.
We use multi-group confirmatory factor analysis with and without alignment to determine the configural, weak, strong and strict MI.
As a secondary outcome, we will examine baseline knee alignment to determine its influence on 18-month disease progression using both semi-quantitative (BLOKS) and quantitative MRI (qMRI).
The Atlantic salmon IGF1 mRNA sequence (GenBank Accession no. EF432852.2) was included in the alignment to determine the putative transcribed regions of the gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com