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The Threaded Blockset aligner (TBA) (Blanchette et al., 2004) also does not require a reference genome for calculating the alignment, but it produces many short local alignments that require ordering against a reference genome.
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Example alignments of intron gains, losses, and alignments that required the outlier A. gam can be found in Additional file 1, Figures S5-S9.
All assemblies were processed using CLC Genomics Workbench version 4.8 (CLC Bio) with an option for global alignment that requires exact matches of the 5′- and 3′-end sequences during assembly.
To test the ability of our approach to find accurate alignments in situations that require warping, we also assemble cases in which we distort the query time series temporally.
Moreover, such nanopore array systems are comprised of two array chips that require alignment, one for circuitry and another for fluidics.
Just a few weeks ago I wrote a blog about customer alignment, something that requires focusing on the needs and wants of customers.
The analysis of this electro-orientation is interesting not only at a fundamental level, but also because of the many applications that require the alignment of these particles.
The use of electrochemical polymerization to functionalize surfaces with MPs provides also an important advantage over lithography-based techniques that require challenging alignment procedures.
A combination of the Swiss-Prot and the TrEMBL components of UniProt provides the source of sequences to prepare the heterofunctional multiple sequence alignments that are required for FDR selection by the Evolutionary Footprinting method [ 26].
We solve the alignment problem using the convolution theorem in harmonic analysis, thus eliminating the need for approaches that require exhaustive angular search, and adopt an iterative approach to alignment and classification that does not require the use of external references.
On closer inspection, we found that our method discovered more accurate alignments in cases that required shorting, whereas COW dominated those trials that did not.
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