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Locus-specific primers flanking (or spanning) at least one intron were designed for opsin and reference genes from alignments of fish sequences (Table 2).
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Increase in the number of robots also significantly alters the degree of alignment of fish motion with a robot.
Degenerate oligonucleotide primers were designed after alignment of various fish, reptile, bird and mammalian AR amino acids sequences.
Thus, we designed primers on alignments of all five fish genomes to increase to success rate of amplification in any teleosts.
Conservation scores based on 8-way mutiple alignments of the five fish species, Homo sapiens, Mus musculus and Gallus gallus were downloaded from USCS Genome Browser Database [ 62].
For that purpose, sequence logos from the amino acid alignments of either the fish or mammalian S100 proteins alone and of a combination of all the S100 proteins were created and used for a comparative analysis.
For the analysis of mammal and fish alignments of complete sequences, sites with ambiguity data were removed (cleandata = 1) since they correspond to indels.
A nice property of vertebrate microRNAs is the high degree of sequence conservation which is found in alignments of man, mouse and fish microRNAs [ 16].
DiRE selects a set of candidate REs from the gene loci based on the inter-species conservation pattern which is available in the form of precomputed alignments of genomic sequence from fish, rodent, human and other vertebrate lineages [ 54].
List of abbreviations and Genbank accession number of fish species used in alignments and phylogenetic analyses (see Table 2).
We base evolvable grouping rules on repulsion-alignment-attraction models which focus mainly on schools of fish and flock of birds [ 15, 25- 28].
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