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Exact(19)
Fifth, sequences were collapsed based on ungapped alignments for which neither sequence was required to be fully aligned.
This optimization was carried out by generating all possible ungapped alignments, for which a score is assigned according to the quality of their corresponding models.
We built the genetic-similarity tree using PHYML from a set of sequences consisting of positions in the locus alignments for which at least two sequences differed from the consensus.
Third, we curated the alignments for which we detected positive selection manually.
This leaves 167 alignments for which the strand prediction of RNAstrand is compared to the strand prediction of EvoFold.
Alignments for which coverage was less than 60% of the shortest sequence and identity did not exceed 80% were removed.
Similar(41)
Our approach starts with assembly-versus-assembly alignment, for which we use the LAST aligner [ 16] with the application of a split-alignment algorithm (Martin Frith, personal communication).
We name "work positions" positions in the multiple alignment for which at least 8 amino acids are aligned.
In this case we consider as correct an alignment where the current range image is attached correctly to an existing cluster (that is, we consider as erroneous only the alignment for which a new cluster is created, rather than all the other images which may attach to it correctly).
Thus, columns in the alignment for which "positional homology" cannot be robustly determined must be excluded from subsequent analyses.
Read-alignments for which mapLength <0.67 or mapQuality <0.75 were discarded.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com