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The L. bicolor protein sequences for allantoinase (EC3.5.2.5), allantoicase (EC3.5.3.4), and urease (EC3.5.1.5) generate similar PSSM alignments consistent with the assigned activities (allantoinase,TIGR03178, E-value of 8.08e-121; allanTIGR02961TIGR02961, E-value of 4.71e-99; and urease TIGR01792, E-value of 0e+00.
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Sequence alignment was performed using the ARB integrated aligner used through an iterative process to generate a nucleotide alignment consistent with the Pfam (36) Fer4_NifH amino acid alignment.
MUSCLE [ 65] was used to produce a multiple sequence alignment of the translated amino acid sequences; a custom java program was used to force nucleotide sequences onto the corresponding amino acid alignment sequences to yield a DNA sequence alignment consistent with the translated sequences.
Family A revealed a mosaic pattern with conserved and variable regions distributed throughout the alignment while family B exhibited a more biased pattern with variation predominantly in the N-terminal half of the alignment, consistent with the analysis described by Tabares et al. [ 44].
Concerning the analyses of the experimental data, our alignment filtering consists of (i) discarding alignments consistent with a SNV from intragenomic origins, (ii) retaining the unique alignments, and (iii) safely analyzing the U and MU regions.
However, this constraint is too tight because it forces all sequences in the subtree to keep the same alignment with the remaining sequences, and may not include any alignments consistent with τ'.
This avoids the problem of A being inconsistent with τ' as long as C is large enough to contain alignments consistent with both τ and τ'.
We now seek a set C that is large enough to contain alignments consistent with τ' and yet small enough for integration and sampling to be computationally feasible.
Another feature of this alignment consistent with our earlier analysis is the observation that Bax α2 and α3, on the right hand side of the peptide in Figure 4a, are more distant from the peptide compared with their prosurvival protein counterparts.
Schlemm's canal endothelial cells showed a statistically significant preferential alignment, consistent with a shear-mediated effect.
The number of osteolytic areas and their sizes in our 40 patients were not related to loss of postoperative AC alignment, consistent with other reports [34, 35].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com