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We aligned each type of tRNA and adjusted them according to their secondary structures (the alignments are the same as those used in phylogenetic analyses), and then the pairwise similarities within each type of tRNA were calculated by pairwise alignment using BioEdit (see Additional file 2).
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The methods for alignments were the same as described for the species tree reconstruction.
Although this general model of one-to-many alignments is the same, our method diverges from that of Ay et al. (2011) after this point.
In Table 2, the upper and lower alignment are the same.
It is also noteworthy that several of the trends observed in sensory-deprived flies (such as AEP, PEP, and footprint alignment) are the same as those observed in faster wild type flies.
Small angle X-ray scattering confirmed that the direction of nanofiber alignment was the same as the direction of shear flow.
The lengths of alignments were also trimmed so all sequences in a given alignment were the same length.
Finally, gap positions are introduced to the candidate sequence so that the final alignment is the same length as the reference database and positional homology is maintained using the nearest alignment space termination (NAST) algorithm.
The protocols of standard PCR reaction, purification, sequencing and sequence alignment were the same (above).
Used alignments and tree topologies are the same as used in codon-based analyses.
For a good separation performance, PM should be consistently above zero, which means the separation alignments are all the same across the frequency bins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com