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The SILVA seed alignments are based on alignments published by the ARB project in 2004.
The multiple alignments are based on the Ciliate EST sequences compared with the full length sequences of the other organisms.
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Nucleotide (codon) alignments were based on the amino acid alignments.
Multiple alignments were based on MUSCLE [61] and edited by hand using Jalview [62], and are available upon request.
Codon sequence alignments were based on the protein sequence alignments.
DNA sequence alignments were based on protein alignments using the Transalign program (Bininda-Emonds 2005).
Alignments were based on the EPO pipeline, where avian alignments included only the three named species whereas mammalian alignments were based on the 13 Eutherian mammals.
DNA sequence alignments were based on codons to retain protein alignments.
TopHat's heuristic filter for spliced alignments is based on this observation.
The alignments were based on BLOSUM62 matrix with gap opening and gap extension penalties -5 and -2 respectively.
23S rRNA sequence alignments were based on the 2D-structure diagrams obtained from Cannone et al. (2002) [ 43].
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