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Multiple sequence alignments analyzed in this study were generated using MAFFT [ 70] using the E-INS_I version (optimal for sequences with conserved motifs and carrying multiple domains) with default parameters.
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In terms of the sequences used, this dataset is a subset of the alignment analyzed in the work of Hwang and Green [ 3] and Margulies et al. [ 12], containing sequences all orthologous to a ~1.9-Mb region on human chromosome 7q31.3 which contains the cystic fibrosis transmembrane conductance regulator gene (CFTR).
File S5 Promultipleultiple sequence alignment for unorthodox aquaporins analyzed in this study.
The results of our experiments show that DELTA-BLAST detects more homologs and provides better quality alignments than do other programs analyzed in this paper.
Only the successful alignment results are shown and analyzed in this section.
Shown in Figure 7 is an amino acid alignment of eight S. aureus strains analyzed in this study (four nasal carriage and four clinical strains) revealing the large degree of homology between the two classifications of strains.
The alignments, matrices of intron presence absence and phylogenetic trees for nuclear-encoded genes analyzed in this work are available at ftp://ftp.ncbi.nih.gov/pub/koonin/MitoCOGs.nih.gov/pub/koonin/MitoCOGs
The factors related to road geometric parameters analyzed in this study are deflection angle, deflection direction and radius of horizontal alignment.
Homology relationships across all genomes analyzed in this study were inferred using the Orthologous MAtrix algorithm (OMA) [ 46] with the following default criteria: alignment length > 60% of the minimum gene length and alignment score > 181 in Gonnet PAM matrix units.
Detailed alignment and annotation information on 14,719 validated SVs from 12 ovarian samples [6 control (whole blood) and 6 ovarian cancer patient samples] analyzed in this study.
Trees from MrBayes and sequence alignments were analyzed in PAML [ 49, 50] to test for evidence of selection acting on sites in the alignments.
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