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MODELLER derives a set of spatial restraints on the structure of the target sequence using its alignment with the sequence of template structure(s).
The mutation types and loci were determined using alignment with the sequence results of M.tuberculosis H37Rv (andC25618, and GenBank accession no. NC_000962) [ 27].
16S rRNA-based identification of four S. gallolyticus subsp. pasteurianus isolates was verified by sequencing of the GroEL gene and alignment with the sequence of the type strain [ 16] showing 100% similarity to the type strain.
Sequencing of the fragment obtained with RUT C30, and its alignment with the sequence of scaffold 2 (Fig. 4) revealed the loss of a 2478 bp fragment which starts 3' of the region encoding the CRE1 zinc finger and reaches into a noncoding region.
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The masked and annotated mouse sequence were then finally used for interspecies pairwise alignment with the sequences from the different species mentioned above using the program PipMaker [ 66].
These consensus structures along with their primary sequence information were then used for alignment with the query sequence.
HMMSplicer's unbiased approach to alignment, combined with the sequence level power of RNA-Seq, has enormous potential for biological inquiry into alternative splicing.
Alignment with the reference sequence for domesticated rice was used to compare the chloroplast genome sequence of wild Oryza species in Asia and Australia [ 19].
Hence, the natural sequence is optimized toward a given coding content and its high quality alignment with the GenBank sequence is not due to chance.
The introns were identified by alignment with the cDNA sequence.
The closest pair is selected, and its three-way alignment with the query sequence is made.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com