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Following standard alignment, only 468 annotated SNPs in 181 genes across all 9 tissues meet a 2.15-fold threshold (corresponding to a 1.5-fold allelic ratio following IUPAC alignment) when requiring at least 10 reads per allele.
A representative set of 47 different cCP sequences (List S1) was used both as a template for all alignments and as an outgroup together, when required, with that of ryegrass mosaic rymovirus (RGMV) as an outlier to the outgroup.
Gbrowse [ 25] was used to display bovine/human alignments and Artemis [ 26, 27] was used for manual annotation of sequence when required.
Use when required.
Feed them when required.
Customize the diagram when required.
Use metric measurements when required.
Reapply as and when required.
Use as and when required.
Report to the Unemployment Insurance Claims Office when required.
The mattresses are designed to ensure spinal alignment when a person is sleeping.
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