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Sequences alignment were made using R-coffee [39] and the alignments inspected and edited using Seaview (v4.0) [40].
After automatic alignment, minor manual adjustments to the alignment were made prior to phylogenetic analysis.
Analysis of sequences and alignment were made by the use of the program ClustalW2 [ 17].
A few manual adjustments to the multiple alignment were made before running Gblocks, which yielded 1,679 positions.
For each non-singleton UniGene, all ESTs in the alignment were made the same length by padding with "N"s where necessary, and all lowercase (PHRED<20) basecalls were replaced with "n"s (multifasta alignment files available at [ 49]).
Postoperatively, when roentgenographic measurements of standing tibial and femoral alignment were made, over 70percentnt of the patients had less than 5 degrees of genu varum or genu valgum remaining.
Similar(54)
Sequence alignment was made with ClustalW (Larkin et al. 2007) and adjusted manually.
A composite of employment and population densities, plus feeder service within ½ mile of the rail alignment, was made.
The accuracy of alignment was defined by the gravure printer since the alignment was made manually without any additional registration system.
Further refinement of the alignment was made manually.
The alignment was made using MUSCLE [35] and was manually adjusted later.
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