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By employing multispecies sequence alignment we identified an ancient (tetrapod-teleost conserved) non-coding architecture within the introns of GLI3.
From this multiple sequence alignment, we identified 330,631 polymorphic positions (14.7% of the total alignment).
In our alignment we identified 73,628 CGIs in dog and 44,518 CGIs in panda (Table 5).
After an initial alignment, we identified many conserved groups without a human ortholog included, even though human orthologs are well known to exist.
Based on this alignment, we identified the DDE catalytic amino acid triad, their associated conserved motifs, and their intervening sequences of amino acids.
From each nucmer alignment, we identified the large differences between reference genomes and de novo assemblies using DNAdiff (Kurtz et al. 2004).
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Using samtools (version 0.1.8 [ Li et al. 2009]) to traverse the alignments, we identified all read pairs for which both members align to the M. guttatus reference genome with a mapping quality ≥29, but have abnormal relative alignment positions (pairs not in the expected orientation and/or an insert size ≥1,000 bp).
Based on multiple sequence alignments, we identified the amino acid positions that are invariant among Arabidopsis Pol I, II and III and S. cerevisiae Pol II, implying that these amino acids are critically important for polymerase structure and function.
Based on sequence alignments, we identified in silico polymorphic SSRs among the four genotypes.
Finally, based on the computed multiple alignments, we identified 118913 non-synonymous point mutations.
Based on these alignments, we identified 80-bp segments with clusters of fixed differences between the two genomes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com