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Using the multiple sequences alignment, we have generated scores on amino-acid matches and mismatches.
The quality of the alignment we have obtained can be tested by comparing the genomic positions of the aligned ORFs.
As the p-value depends on the length of the alignment, we have used the linear regression (−0.2837' · length of alignment + 66.0155)/100 to adjust it to different satellite lengths.
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"The alignment we've already established with Mitsubishi will greatly enhance our plans to maintain Cutbank Ridge's leadership position among the most cost competitive resource plays on the continent".
Since the ion-selectivity filter in OR83b was not obvious by mere sequence alignment, we had built two models (Models 2 and 3 involving IC2 and EC3 as the possible selectivity filter region, respectively) and analysed the electrostatic distribution of the protein around these two loops to recognise the likely pore region.
To re-align SVRs in quest of improvement of alignments, we have adapted our previous approach [41], [45].
Through exploratory analysis using simulated data and profile-profile alignments, we have found that these locally optimal profile models often align with the correct profile (from which the data were simulated) after local shifts of the model positions.
To aid in use of progressiveMauve alignments, we have developed an interactive visualization program that can present a complex alignment in a meaningful and easily understandable visual paradigm.
Using these set of alignments we have identified 288,957 high quality single nucleotide polymorphisms and 1,480 indels.
After traversing all alignments, we have a set of conserved syntenic regions, on which we can impose a minimum member limit (typically three pairs) to removed spurious regions.
Using the locally collinear blocks generated from the whole-genome alignments we have also analyzed the existence of recombination events by building ML trees of randomly selected regions (Additional file 1: Figure S2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com