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The resulting alignment was verified using AlnEdit and converted into aligned FASTA-format (for input to HMMaccel).
Articular involvement, when present, was accurately reduced; restoration of length and correct alignment was verified with an image intensifier, and the fracture was fixed with the plate.
Each alignment was verified by eye and compared with secondary structures (16S rDNA) [84].
Alignment was verified manually, matching artifacts deleted, and misalignments corrected.
The femoral and tibial reference arrays were retained until the cement had fully set, and were removed after the alignment was verified under navigation.
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Once proper axial alignment is verified, the proximal end of the tibia can be cut at usually 10 mm depth from the unaffected tibial condyle.
Alignments were verified and adjusted manually.
Alignments were verified by using SeaView (http://www.molecularevolution.org/software/alignment/seaview).org/software/alignment/seaview
Allelic variations noted from multiple alignments were verified by inspecting the respective electropherograms.
The LTR alignments were verified using ClustalX [ 15], after manual editing as necessary (see Supplemental data).
Alignments were verified and corrected manually using the MEGA 4.0 program [ 40].
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