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The domains were aligned individually, and the combined alignment was used as input for MEGA2.
The RDP alignment was used as a profile to align GenBank sequences using ClustalW v1.83 [ 30].
The "Align manually" option, which allows the user to individually set the time alignment, was used.
Finally, this alignment was used to construct a phylogenetic tree using the same previously mentioned software.
18 placental mammals from the Multiz 28-way multiple alignment was used as input.
The full alignment was used in the case of the ERF-B3, CDF and ID families.
The resulting amino acid alignment was used as a guide to obtain the corresponding nucleotide alignment.
This alignment was used as a reference to which the alternative alignments are compared.
Where appropriate, a longer alignment was used for the phylogenetic analysis (see supplementary data).
The target-template alignment was used to build the model in Swiss Model [80], [81].
Given the problematic nature of repeated sequences, the best initial alignment was used as a starting point only.
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