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The alignment was initially generated using CLUSTALW and curated in Bioedit v. 7.0.4.1.
Sequence alignment was initially performed using MUSCLE 3.8.31 [ 38] in the multiple alignment routine followed by manual adjustment in Se-Al v2.0a11 (http://tree.bio.ed.ac.uk/software/seal/).ac.uk/software/seal/
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Briefly, regions of ambiguous alignment were initially excluded from preliminary analyses (Step 1 4, flowchart for our methodology on Fig. 6).
A total of 13329 alignments were initially constructed.
Sequence alignments were initially performed with ClustalW [ 34] in BioEdit version 6.0.6 [ 35] and adjusted manually.
These alignments were initially analyzed using DnaSP version 4.0 [ 78] to identify polymorphic sites in all three alignments.
Multiple sequence alignments were initially made using the program ClustalX version 1.81 with default gap penalties [ 38].
To avoid incorrect indels in the codon alignments, multiple sequence alignments were initially performed with amino acid sequences from each gene cluster, followed by conversion to the corresponding codon alignments using custom Perl scripts.
I suspect that Apophis' apocalyptic appeal comes in part because of a comsic alignment that meant it was initially thought to be most likely to impact on a Friday 13th.
Multiple alignment of each dataset was initially carried out using MUSCLE, version 3.8.31 [ 63].
An alignment of the protein sequences was initially generated by MUSCLE software and refined manually.
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