The phrase "alignment was first" is correct and usable in written English.
You can use it when discussing the order of operations or priorities in a specific context, particularly in fields like project management or design.
Example: "In our project timeline, alignment was first before we moved on to the execution phase."
Alternatives: "alignment came first" or "alignment took precedence".
The query sequence and template structure alignment was first performed using the Modeller Align module, and then manually inspected to ensure the best alignment for generating a pool of 2000 models.
Alignment was first performed at the familial level by aligning all the well established monophyletic groups using T-coffee (version 5.05; [ 63]).
Whole-genome alignment was first performed with Mauve program55 and polished with CLUSTAL W aligner and finally manually inspected.
Input (1): for each locus, the multiple sequence alignment was first filtered to remove ambiguous or incomplete codons, as well as premature stop codons.
The idea of signal alignment was first introduced for MIMO Y channels[9].
A proposal for a highway along a similar alignment was first proposed in 1955, as part of a network of arterial roads under a metropolitan-wide plan produced for the Western Australian government by Gordon Stephenson and Alastair Hepburn.
Briefly, the alignment is first divided horizontally into sequence subfamilies using Secator [61].
The sequences in the complete alignment were first divided into more related subfamilies using the Secator program [61].
A multiple sequence alignment is first performed using the target sequence and a large number of homologous sequences.
Portions of ambiguous alignment were first adjusted manually and then masked to remove columns in the alignment with > 30% gaps prior to phylogenetic analysis (Additional file 13).
To create a consensus or profile, each column of the multiple alignment is first examined for the number or fraction of gaps, to determine if the column should be considered part of the profile (or consensus), rather than an insertion column.
