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The human-mouse alignment was created by aligning both genomes using blastz [ 104].
Multiple alignment was created by aligning all sequences against Hidden Markov Model GTP_EFTU from Pfam [ 105] using program HMMALIGN [ 18].
A multiple sequence alignment based on the structure alignment was created through a companion program, Match → Align.
A final alignment was created in which the POMGnT1 sequence was aligned with the sequence of the homology model.
The sequences were aligned with publicly available Emu sequences for each region and a concatenated alignment was created for the control and COI regions.
For each DNA sequence, a multiple alignment was created by Clustal W Thompsonn et al. 1994).
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500 resamples of the alignment were created with Seqboot version 3.67 [ 50].
This alignment is created using the Matt program (Menke et al., 2008).
Three types of alignment were created: A: duplicate sequences from the array were combined with all other gene family members.
Unrooted tree files for each alignment were created using a standard mammalian species tree [ 87] ((human, chimpanzee), (mouse, rat), dog).
In such analysis, the paralogous proteins for each species were pairwise aligned using MAFFT v6.6 [ 47], and subsequently the corresponding codon-alignment was created according to the resulting protein alignment using a custom PERL script.
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