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In the analysis, the new sequences were compared with consensus sequences (50% threshold) representing the HIV-1 variants from the same alignment used for phylogenetic tree analysis.
The MLSA rarefaction curve was constructed with the same concatenated nucleotide alignment used for phylogeny, while the CRISPR rarefaction curve was constructed based on the colored representation of the CRISPR loci spacer arrays.
In the structural alignment used for modeling of UNC-8 to 3HGC, the only non-proline cis-peptide in 3HGC which aligns to the sequence of UNC-8, aligns to a proline in the UNC-8 sequence and was maintained.
Protein sequence alignment used for HMMER3 search.
Schematization of KatG protein alignment used for the phylogenetic analysis.
Additional file 9: Sequence alignment used for the reconstruction of the nc H4/H3 tree.
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By placing one of the quarterbacks at least seven yards behind the line of scrimmage, and no one under center to receive the snap, the A-11 qualifies as a scrimmage kick formation — the alignments used for punts and extra points.
For more information about the multi-species alignments used for these fRNA prediction programs, see Text S1.
The alignments used for this purpose were retrieved from the European rRNA database [25] which aligns bacterial and archaeal rRNA sequences with eukaryote sequences.
Repeat sequences spoil multiple genome alignments used for the computation of PhastCons scores, therefore we limit this analysis to 298 431 sites within non-repetitive sequences.
The alignments used for the analyses within each group of homologues represent a concatenated sequence of the different conserved motifs found within each group (Figure 2).
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