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The trimmed sequences from each barcode bin were aligned using the NAST alignment tool (http://greengenes.lbl.gov) [32].
Approximately 1.8 kb of genomic sequence from the Agrp locus of 10 mammalian species, including the transcription unit and the proximal conserved region, were aligned using the multi-Lagan alignment tool (http://lagan.stanford.edu) [17].
Both cysteine domains were aligned using the European Bioinformatics Institute ClustalW online alignment tool (http://www.ebi.ac.uk/Tools/msa/clustalw2/), and the TEXshade alignment shading software (Beitz, 2000) was used to create the graph.
These sequences were aligned by BLAT (BLAST-Like Alignment Tool, http://genome.ucsc.edu/cgi-bin/hgBlat) versus the 2007 release of the Mus musculus (mouse) genome, to identify the limits of the mouse 3'RR contig (chr12 114,459,657-114,497,890 in mmu9 draft; size 38234 bp).
Briefly, PlantGDB-provided component sequence files of sequences that were used to generate PUT sequence assemblies and subsequence files of near exact matching duplicate sequences that were excluded from the assembly process were aligned to the PUT sequence assemblies using the vmatch alignment tool (http://www.vmatch.de).
The sequence tags were then aligned to the rat genome (assembly rn4) with the Bowtie alignment tool (http://bowtie-bio.sourceforge.net/index.shtml).shtml
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To decrease errors in EST alignments and determine the chromosomal loci of each gene, we localized ESTs to genomic sequences using BLAST-like alignment tools (http://genome.ucsc.edu).ucsc.edu
The presence of a potential miR-34a consensus site (7 mer) in the 3′ UTR of the Bax mRNA was established using independent bioinformatic alignment tools (http://www.microrna.org and http://www.targetscan.org).org
To facilitate structure-functional analysis of genetic variations in kinase genes, all crystal structures and mutational models were structurally aligned using a java-based multiple alignment tool STRAP (http://www.charite.de/bioinf/strap) and TM-align algorithm [124].
Upstream and intronic genomic sequences for human and mouse Mef2c were obtained from both the Ensembl and UCSC Genome Browser web sites and were aligned using the multisequence local alignment tool MULAN (http://mulan.dcode.org/).org/
Multiple sequence alignment of MAPKs were carried out using the multiple sequence alignment tool Multalin (http://multalin.toulouse.inra.fr/multalin/) to identify the conserved domains.
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