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We carefully inspected the alignment to verify its consistency with the literature.
We visually inspected the alignment to verify that key sequence motifs (e.g., HRD and DGF motifs) and core hydrophobic residues are aligned correctly.
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Before attempting a detailed phylogenetic analysis of the Arabidopsis PLDs, we used a combination of computational tools, comparison with cDNAs/ESTs and information from protein alignments to verify the exon-intron structures proposed by AGI annotators (see Materials and Methods).
Examine the alignment page to verify that it displays a series of marks that are black and blue in color.
The first type of alignment was used to verify the monophyly of the class 4 HDACs and thus to ascertain that we had identified bona fide class 4 HDACs (see Additional File 1).
For example, mismatch profiles are given for high-and low-quality alignments allowing users to verify whether poorly mapped reads contain a specific collection of mismatches.
The completed measurement based on a Laser Tracker and a Micro Alignment Telescope was conducted to verify its correctness.
The resulting transformation matrix was then applied to each participant's beta map as well as to the MTL masks in order to verify alignment precision.
We compared sequenced products to known esociform RAG1-I2 sequences on GenBank with the basic local alignment search tool (BLAST) to verify amplification of the correct genomic region.
Reviewer 1 The lack of displaying representatives from the other subfamilies in the output alignment makes it difficult to verify the predicted status of subfamily-specific conservation.
Manual examination of BLAST alignment results was performed to verify residue conservation at and around the active site, as well as exclusion of proteins with additional homologs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com