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The program ClustalW2 [ 31] with default settings was used to perform multiple sequence alignment to use as input for PHYML.
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Dr. Esquenazi and colleagues are also developing a portable alignment system for prosthetists to use in their offices.
To that end, forced alignment is used to align text and speech features using the speaker-dependent HMM models.
Alignments produced by HMMsearch (25) were parsed using Perl scripts; this was followed by manual alignment to the HMMsearch alignment using GeneDoc (27).
Reverse sequences were reverse complemented (http://bioinformatics.org/sms/rev_comp.html) prior to alignment using ClustalW.
In fact, pair-wise alignment is used to compare and to cluster sequences.
Peak alignment is used to assign corresponding peaks to the same analyte in different samples.
Gene functions were assigned according to the best alignment attained using BLASTP to the UniProt database (including SWISS-PROT and TrEMBL databases) and INTERPRO scan.
Finally, this alignment was used to construct a phylogenetic tree using the same previously mentioned software.
The target-template alignment was used to build the model by satisfaction of spatial restraints.
The target-template alignment was used to build the model in Swiss Model [80], [81].
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