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We identified 2,716 pairs of Limulus markers that can both be placed on the map and have their best translated alignment to the same Ixodes scapularis gene.
We used the designation "paired BLAST hit" (PBH) to describe an instance when both BESs from a particular BAC exhibited significant alignment to the same chicken chromosome in the same orientation regardless of the distance between hits.
Application of other existing programs aimed at long gap alignment to the same set of contigs from the 1000 Genomes Project revealed a substantially higher SV confirmation rate when using AGE, thereby suggesting its superior performance in determining SV breakpoints (Supplementary Material).
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To maximize the identification of short CSBs between evolutionary divergent orthologs, EvoPrinterHD generates 3 different input reference DNA vs. test genome BLAT alignments to the same aligning region using the three indices described above.
Duplicate alignments (reads aligning to the same genomic position) were removed using SAMtools [ 13].
Next, sequences that were not aligned were subjected to translated sequence alignments to the same viral references, which were translated in six frames.
For inclusion in Tables S1 and 4 we applied an arbitrary maximum expect value of 10−4 and an arbitrary minimum of 70% identity at the nucleotide sequence level or two or more alignments to the same locus/function in different plant species.
If a read had multiple alignments to the same amplicon, the entire region spanning across multiple alignments was kept.
Only alignments to the same chromosome in N2 and Hawaiian and on the same strand were retained.
bMultiple alignments to the same A. thaliana protein were condensed to provide a non-redundant estimation of representation of the A. thaliana proteome.
The probability of there being a better match for the guide sequence to a given Cas9 target sequence was calculated as the frequency of Cas9 target sequences with better alignments to the same guide sequence, amongst 10,000 randomly selected Cas9 target sequences.
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