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Nucleotide sequences were aligned and gapped manually using Bioedit software version 7.0.9 (Tom Hall, Ibis Biosciences, Carlsbad, CA), based on the protein alignment to retain codon positions.
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A plurality of magnets are arranged on the camera module adjacent to the alignment feature to retain a detachable lens on the housing and engage the alignment feature to align the detachable lens with the optical axis.
Although the general approach seems relatively straightforward, many details remain to be resolved in this ongoing project, including how best to splice the resulting alignments of gene clusters into a whole-genome sequence alignment so as to retain the existing alignments outside of gene clusters.
We did this by performing BLASTN searches between the A. mexicanum and A. t. tigrinum assemblies, and the resulting alignments were filtered to retain only those alignments between sequences that were one another's reciprocal best BLAST hit.
Resulting alignments were filtered to retain only the best alignments, requiring at minimum 80% of the trimmed sequence aligning with at least 90% base pair identity.
The alignment is set to retain maximum equivalence in TM, and important motifs such as DRY and NPXXY (in TM3 and TM6, respectively) were kept aligned in both query and template.
The HMM alignments were trimmed to retain the conserved regions (HMM "match states") using BBEdit.
To focus on the taxa with the most incongruence, and to simplify the comparison, alignments were pruned to retain JD317 and eight isolates with the highest genetic similarity to it.
After identifying Wukong elements from NGS data, the 150-bp sequences immediately flanking the insertion sites were extracted from the B73 reference genome and grouped into a database, which were used as queries to search NGS short reads of 76 resequenced accessions one by one; alignments were trimmed to retain the matching portions and then were extracted from the corresponding databases.
Thus, not only do cells that are aligned with nanoridges tend to retain their alignment, but cells that are perpendicular to nanoridges tend to turn to improve their alignment.
DNA sequence alignments were based on codons to retain protein alignments.
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