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Positions with >80%% gaps were removed from this alignment to perform phylogenetic analysis and Sd/Nd analysis to avoid excessive gap penalties and prevent analysis errors caused by gaps.
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We therefore used T-COFFEE [ 16], a multiple sequence alignment tool, to perform sequence alignment of these amplicons for scoring the sequence similarity.
PyNAST uses UCLUST (Edgar, 2010) instead of BLAST, whereas mothur relies on its own implementations of a k-mer search to select the reference sequence and a Needleman Wunsch type alignment algorithm to perform the pairwise alignment.
The alignments used to perform the phylogenetic analyses are provided in supplementary data sets S4 and S5, Supplementary Material online.
The web site allows users to view contig assemblies along with their read alignments, and to perform BLAST searches against assembled sequences.
We do not use the alignment score to report best hits because the alignment ratio proves to perform better.
Limited by the range resolution, the motion estimate from range alignment is insufficient to perform an optimal phase correction.
After the alignment, we proceed to perform a linear dimensionality reduction of the set, using the SVD.
Alignment is difficult to perform because size variations occur near their 5' ends.
To reduce the number of false indels arising from wrong alignments, we employed sequence alignment programs based on structures to perform pairwise alignments of non-redundant structures in each family.
Sequence alignments were built to perform the positive selection test.
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