Exact(5)
Transitivity over each hierarchy extends the guide alignment to all pair-wise comparisons in multi-domain families.
Annotation of each mitochondrial genome was done by alignment to all octocoral genomes available in GenBank (table 1) with the aid of the software CLC Main Workbench.
Therefore, we developed a new, cutoff independent approach to assign critical rare codons which compares the observed codon composition of one column in a multisequence alignment to all possible, alternative combinations of synonymous codons.
OlfC gene sequences were considered intact if they 1) begin with a signal sequence, 2) end in a stop codon after the seventh predicted transmembrane domain, 3) show significant alignment to all six stereotypical OlfC exons, and 4) possess no internal frame shifts, significant deletions (excluding those caused by gaps in the genome assembly) or stop codons.
NGS reads were filtered for exclusion of human sequences using Bowtie2 high-sensitivity local alignment to the human hg38 reference database at default parameters followed by BLASTn alignment to all primate sequences in NCBI nt at an e-value cutoff of 10−8 prior to deposition into the NCBI SRA database.
Similar(55)
Within each ortholog triplet, all human frame +1 and -1 tORFs were compared in pairwise global alignments to all those computed from mouse and from rat.
Subsequently, the human genomic regions that possessed the orthologous pairwise alignments to all the three great ape were extracted and multiply re-aligned with the corresponding sequences of the three apes with MAFFT (Katoh and Toh 2008).
We used the GLAM2 parameter that forces the alignment to involve all input sequences.
The other two alignments are matched negative controls generated by shuffling the order of positions in each observed alignment to remove all structural information.
Initially a custom Python script (available at < http://prodata.swmed.edu/jrs/maf-stachy2/>) was run on the whole-genome alignment to identify all candidate clusters.
Despite that, our method (like many other genome aligners) applies a global alignment algorithm to all inter-anchor segments, navely assuming that homology exists.
More suggestions(1)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com