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In cases of single pair matches, a merge was performed if the alignment to a sequence contig indicated an overlap.
When the analyzed 3D structures correspond to remote homologs, mapping the 3D alignment to a sequence alignment is often tricky since a small inaccuracy in the 3D structure superimposition can lead to the assignment of a residue of a sequence with the neighbor of the true corresponding residue in the other sequence.
If two sequences participated in a pairwise alignment above a pre-selected threshold quality, they were put into a "bin"; additional sequences were added to the bin if they had a sufficient pairwise alignment to a sequence already in the bin.
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Slider is an application for the Illumina Sequence Analyzer output that uses the probability files instead of the sequence files as an input for alignment to a reference sequence or a set of reference sequences.
The InSites algorithm compares sequences in an alignment to a reference sequence and shows each phylogenetically informative site, defined as a site at which more than one sequence differs from the reference sequence.
Long subsequences were selected from across each full sequence that could seed alignment to a target sequence with any degree of overlap.
This technique allows the production of extremely long (hundreds of kbp) restriction maps, which to date has been applied to, e.g., alignment to a reference sequence to identify structural variations in bacteria [ 19– 22] and in humans [ 23– 25].
Algorithms are classified by whether they use an alignment to a reference genome sequence, or whether they assemble the reads into full transcripts de novo by identifying overlapping reads.
To be considered a match, array query sequences were required to have >50% sequence alignment to a model RefSeq transcript hit and >98% identity for the aligned sequence.
Here we present a draft bighorn sheep WGS created by cross-species alignment to a domestic sheep reference sequence.
QRNA predictions were filtered by alignment to a variety of coding sequence databases using BLAT [ 40] with a default score cutoff of 30 ([alignment length] – [number of mismatches]) and a minimum sequence identity of 60% (-minIdentity = 60).
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