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To construct a reference-free multiple sequence protein alignment, the sequences were aligned using Muscle v3.7 software under default parameters (Edgar 2004).
In pair-wise alignment, the sequences among the same species had high homology of over 90%.
After the alignment, the sequences of graphemes and phonemes have the same number of segments.
After alignment, the sequences were edited manually.
After alignment, the sequences were analyzed by a custom program written in perl and matlab.
Following the alignment, the sequences were submitted to WebLogo Berkeley (http://weblogo.berkeley.edu/logo.cgi) to generate a sequence logo.
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In the final alignment, the sequence of 1HGJ (HA HA structure) was included to follow the H3 numbering.
Using these alignments, the sequences were threaded onto the templates using the program DEEP VIEW [97].
After alignment, the concatenated sequences become 2173 bp long.
ClustalW was used to perform multiple sequence alignments of the sequences associated with selected reads and assembled reads into contigs.
CLUSTALX was used for constructing multiple alignments of the sequences.
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