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Based on this EST-genome sequence alignment, the average intron length was estimated as 52 bp.
The averaging procedure and the complete alignment procedure are very robust: after the alignment the average trajectories were reproducing very closely the ground truth trajectory.
To validate the quality of our alignment, the average number of nucleotide substitutions (Dxy; [ 70]) was estimated for 100 kb non-overlapping windows (Additional file 11: Figure S6) and genome-wide patterns compared to previous literature [ 71].
Using 12 threads, for the SE PEE) alignment, the average speedup is about 11.4 (11.3) for CUSHAW2, 10.6 (10.6) for BWA-SW and 8.7 6.3) for Bowtie2, whereas GASSST only has an average speedup of about 2.2 for the SE alignment.
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When the N-terminal chloroplast peptide signals are removed from sequence alignments, the average identities reach the scores of 79% (SDR152C) and 84% (SDR87D and SDR 52E).
The likelihood and the branch lengths shown are based on the concatenated super gene of the 988 individual four-species alignments; the average values of the branch lengths from the individual genes are highly similar to these results.
Each generated sequence was considered to be an ancestral sequence, and the process of column evolution in each column of the generated sequence was modeled by random generation of 32 amino acid residues to obtain the same depth of the generated multiple alignment as the average depth of multiple alignments in our clustering procedure done on BLOCKS.
Furthermore, CUSHAW2 is on an average superior to all other evaluated aligners in terms of both recall and precision for the SE PEE) alignment, where the average recall is ~89.92% (90.75%) and the average precision is ~97.46% (98.28%).
It is an iterative procedure that repeatedly builds projection traces from cluster seeds and refines the micrograph alignment until the average peak number in the traces becomes maximal.
Sliding windows of 8 kb were passed along the alignment, and the average values were plotted using Circos [ 61].
(E ) A diagram summarizing the steps that led to the spatial and temporal alignment of the average trajectories.
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