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We find evidence for this inadequacy by comparing the alignment summary statistics for B6 and CAST liver samples aligned to their respective strain-specific transcriptomes.
Alignment summary statistics are presented in Additional file 3. Aligned sequence reads and a list of genomic features (Gmax_189_gene.gff3, www.phytozome.net) were input into HTSeq to generate read counts using the htseq-count and –m union options.
Alignment summary for the CLC and Velvet/Oases assemblies.
These alignment summary reports were generated by a 'flagstat' command of SAMtools (Version 0.1.19-44428cd).
EnsemblPlants provides highly interactive web interfaces to show phylogenetic tree and alignment summary graphically, but fails to show alignment in details.
The Picard command CollectMultipleMetrics was employed to each of the merged bam files to provide information on alignment summary metrics, insert size metrics and quality by cycle metrics.
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Alignment summaries were output by BLASR and LAST and 'pileup' files were generated using Samtools [ 25].
Alignment summaries for all presumptive Ambystoma-vertebrate orthologs are provided as supplementary data [see Additional file 1].
As our software makes no assumptions based on prior biological or biochemical knowledge, the end-user is able to navigate the full set of returned alignment summaries (strength of hit as E-value and bit score, subject accession numbers, etc).
Additional file 1: Alignment statistic summary of all 15 samples signal-end reads mapped to UCSC H. sapiens reference genome (build hg19) using Tophat alignment program.
Additional file 1: Figure S6 graphs the loadings for these PCs as they depend on number of species, depth of alignment and summary statistic; Additional file 1: Figure S4 and S5 depict the correlation matrix and a hierarchical clustering of these variables, respectively.
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