Your English writing platform
Discover LudwigExact(60)
A phylogenetic tree was generated by RaxML based on the sequence alignment shown in panel (b).
Topology 1b is the most parsimonious evolutionary reconstruction for the DNA alignment shown in Fig. 1a.
The vertical axis illustrates various SSD values corresponding to the stations of the compound alignment shown horizontally.
Firstly, the pro-smtg gene nucleotide sequences of the four mutants showed no differences from the original sequence of the native enzyme from the wild-type strain, as indicated by gene cloning and sequence alignment shown in Additional file 1: Figure S3.
The first alignment shown on the left keeps 18 areas contiguous whereas the second alignment shown on the right keep 22 areas contiguous.
The final model (shown in Figure 4) was generated according to the target/template alignment shown in Figure 5.
The low sequence similarity is also indicated in the corresponding multiple sequence alignment shown in Figure S1.
Our sequence comparison (e.g. Helix B domain alignment shown in Figure S6) clearly highlighted only a few amino acid substitutions which appear to be species-specific.
After manual addition of sequences obtained from other primates by PCR, all exon alignments were concatenated to produce the final alignment shown in Figure S1.
Using the program SDM, we identified the mutations that would significantly alter the Gibbs Free Energy (ΔG); the positions are highlighted in the alignment shown in Figure 1.
The multiple alignment shown in Fig. S2 for the β-barrel domains was used to derive average hydropathy, amphipathicity and similarity plots (Fig. 5).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com