Exact(12)
We suspect that in many cases such alignment segments are in fact poly(A) tails that should not have been aligned.
The maximum false positive score, based on ungapped alignment segments from permuted versions of the data set, was 13.6±0.8 (n = 10).
Alignment segments predicted to contain conserved RNA secondary structure were discarded unless they contained at least 20 bases of D. melanogaster sequence and sequence from at least four other species with gaps in no more than 7.5% of predicted base-pairs.
Those with greater CDS coverage than peptide coverage were identified as having additional out-of-frame alignment segments.
In contrast, the BLAST raw score is directly derived from alignment segments can eliminate this artifact [ 27].
Alignment segments containing indels polymorphic in D. melanogaster were realigned with MUSCLE (Edgar 2004) to increase the quality of alignment with D. sechellia and D. erecta.
Similar(47)
The alignment segment shown (Fig 3A) does not allow a clear assessment of homology of the residues involved.
Our analysis showed minor differences in the target alignment segment, where a consensus sequence of Rx[KR]R is favored.
This method involves 3 steps: first, the nucleotide substitution model parameters and branch lengths are obtained by ML given the alignment segment and the neighbor-joining tree.
The trees generated from each non-recombinant alignment segment were inspected, and the strain with highest observed discord between trees was removed.
Just outside the substrate alignment segment, at the P5, acidic residues are preferred in all groups of putative targets, except for PCSK7.
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