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Poor electron optical alignment, drift, detector noise, magnification variations, inaccurate defocus determination, errors in alignment, sample heterogeneity, flexibility, incomplete angular coverage, and radiation damage can all combine to give dramatic falloff of the high-resolution information.
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Multiple sequence alignment samples can be summarized in several ways.
A "Noah's Ark" dataset was also analysed which comprised 14 eukaryotes (indicated by asterisks in Fig 1) sampled from each major taxon and included 120 sequences and an alignment sampling of 307 amino acid characters.
To further investigate the evolutionary ancestry of the gene fusion character, we calculated individual phylogenies for the three pterin biosynthesis domains with both comprehensive and reduced taxa alignment sampling.
We developed a maximum likelihood model fitting procedure to a collection of HIV-1 alignments sampled from different viral genes, and inferred two empirical substitution models, suitable for describing between-and within-host evolution.
Our method for sampling alignments samples from a distribution η that approximates the correct distribution π but does not match exactly [ 24].
The Context program, which uses context-sensitive indels, was trained on a separate set of 200 alignments sampled from our model.
The entire device is fully automated from optical alignment to sample injection, readout and analysis.
After alignment, the samples were divided into the appropriate groups: furosemide before (normal urine samples) and after (urine samples collected after gavage); hydrochlorothiazide before and after; and spirolactone before and after.
MG participated in the sequence alignment and sampling procedure.
We obtained between 45 and 85 million unambiguous alignments per sample.
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