The phrase "alignment require" is not correct in written English.
It seems to be missing a subject or an article, making it incomplete.
Example: "The alignment requires careful attention to detail."
Alternatives: "alignment needs" or "alignment demands".
Other applications of sequence alignment require different algorithms.
Furthermore, methods based on genome alignment require normalization by genome size in order to estimate taxonomic abundance without bias [ 7], something that is not possible to estimate for uncharacterized species.
For example, annotations made with the ISA GO evidence code ('inferred from sequence alignment') require the inclusion of a stable database identifier that identifies the similar gene/gene product.
We generated the true INDEL call set by two steps: (1) used GATK UnifiedGenotyper to call INDELs from the BWA-MEM alignment, (2) performed manual inspection on the large INDELs from the Bowtie2 alignment (require at least 25% of the reads supporting an INDEL) [ 43].
The tree alignment requires us to align each state with at most one other state, but not two or more.
When CECs on vertically oriented NGs were exposed simultaneously to a horizontal EF, they aligned more robustly than to either cue alone and the enhanced alignment required rho signalling.
We used FASTA [ 81] to align GLEAN coding sequences with RefSeq coding sequences where a perfect alignment required 99% identity over the entire lengths of both sequences.
Determining the alignment requires transmitting light through the fibers, typically between two points each several miles from the splice.
The impact of the ProDisc-C (Synthes Spine, Paoli, PA) on in vivo kinematics and sagittal alignment requires further assessment.
STI structures are the first encountered in case of editing through the chip backside and accurate CAD alignment requires trenching until the lower STI edge becomes visible.
The longer conversion times for the shorter spacer are attributed to lower freedom of movement within the monolayer to achieve the spatial alignment required for the topochemical polymerization.
