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For these N and Sb contents, the system will still be in the type-I band alignment region [12].
In addition to this criterion, to be included in a homolog group the difference in length between the subject protein and query protein had to be <10%, the alignment region had to be at least of 80%, and there had to be a at least 50% similarity of both query and target sizes.
For a few subfamilies, some hits were restricted to a short alignment region with fuzzy limits.
Further detailed examination was done of the alignment region immediately surrounding the PRE.
Sequence divergence was calculated using the alignment region with sequence in all species compared.
In the output BAM file, Socrates maintains the associations between the new alignment region of the long soft clipped sequence, which we refer to as the re-aligned locus, and the original alignment region, which we term the anchor.
Similar(32)
All HT-based alignment tools use the same seed-and-extend strategy [ 11, 12], which proceeds by searching for candidate alignment regions (CARs), aligning each location, and reporting the best alignments.
We identified proteins with two or more non-overlapping alignment regions where each region aligned separately to smaller homologs.
Protein models were aligned using MUSCLE and low-quality alignment regions were removed by TrimAl (Edgar 2004; Capella-Gutierrez et al. 2009).
First, each gene-set was separately aligned using muscle 3.6 [ 33] and ambiguous alignment regions were removed using Gblocks (version 0.91b) [ 34] with no gaps allowed.
Amino acid replacements were calculated for all gap-free alignment regions of >100 residues and also for fully aligned sequences including gaps.
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