Exact(3)
The alignment rates of the reads generated from the 12 samples which mapped against the pseudo-reference range from 67.21 to 85.31 % while the alignment rates range from 66.11 to 84.19 % if the reads were mapped against the original MSU7 cDNA sequences.
Section 4 provides a dataset analysis, which reports alignment rates of Bowtie2, BWA, and BLASR on corrected and uncorrected PacBio datasets.
The low alignment rates of Bowtie1 means that methods have available only a tiny fraction of the useful data, leading to less accurate results.
Similar(5)
Unfortunately, despite these steps, the alignment rate of each aligner was significantly lower than expected, so to offset this, the fastx toolkit was used to filter out low quality reads (Table 1).
On average, 26 million reads were aligned per sample with a mean alignment rate of 97%.
On average, 36 million uniquely aligned reads were obtained per data set, representing an average unique alignment rate of 76%.
Using Bowtie 2 (Langmead and Salzberg 2012), reads were aligned to the reference genome sequence UMD 3.1 with an average alignment rate of 97.6%, that covered 99.23% of the genome (supplementary table S1, Supplementary Material online).
We retained alignments with an alignment rate of more than 70% and a mapping identity of more than 40%.
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