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We defined a number of criteria to identify orthologous sequences including minimum alignment length (200 bp), minimum sequence identity (90%), and minimum alignment proportion (> 80% of shorter sequence).
We also reported, the number of contigs, coding sequences, and genes that had a significant hit (e-value < 1e-10) independently of the alignment proportion.
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Gblocks, ver. 0.91 [ 61] was used to identify unambiguously aligned proportions of the alignments.
Ambiguously aligned proportions of both alignments were excluded using Gblocks version 0.91b [ 61] with default block parameters.
Concerning the HKA as parameter for good mechanical axis alignment, the proportion of patients with a deviation of ≤3° of normal was 81.4% postoperatively and for DFA and PTA 94.7% and 92.0%, respectively.
For the wallaby-referenced alignment, the proportion of bases that are conserved in each sequence class and the distribution of conserved bases across classes are shown in Figure 3.
In these alignments, the proportion of mismatches was highest at the ends of these alignments where there are typically lower numbers of sequencing reads (Fig. 3).> -wrap-foot> Summary of BLASTN searches of the transcriptome using published sequences from five protein-coding genes as queries.
For all patients, the deviation (continuous variables) from the expected ideal alignment and the proportion of patients with deviations ≤1, 2, 3, and 4° from the expected ideal value was calculated.
Differences in varus/valgus alignment and male/female proportions were compared in each group and the differences did not correlate with LRR rates.
To minimize the amount of missing data in the resulting alignments, a small proportion of nucleotides at the 5'- and 3'-ends of the matrix for these four regions were excluded prior to analysis (to a point where at least 50% of sequences were full length on each edge).
The effect was more drastic for Type II New genes; only 0.3% of Type II New genes overlapped a D. melanogaster homolog alignment, while the proportions were 4.9% and 10.9% for Type I New and Previously Known genes, respectively.
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